dc detergent compatible protein assay 5000112 bio rad Search Results


dc protein assay kit  (Bio-Rad)
96
Bio-Rad dc protein assay kit
Dc Protein Assay Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pmc08363747-387-6-10?v=Bio-Rad
Average 96 stars, based on 1 article reviews
dc protein assay kit - by Bioz Stars, 2026-08
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dc protein assay  (Bio-Rad)
97
Bio-Rad dc protein assay
Dc Protein Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pmc03598628-80-8-11?v=Bio-Rad
Average 97 stars, based on 1 article reviews
dc protein assay - by Bioz Stars, 2026-08
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04693116001 dctm protein assay kit ii biorad 5000112 blotting grade blocker non fat dry milk biorad  (Bio-Rad)
96
Bio-Rad 04693116001 dctm protein assay kit ii biorad 5000112 blotting grade blocker non fat dry milk biorad
04693116001 Dctm Protein Assay Kit Ii Biorad 5000112 Blotting Grade Blocker Non Fat Dry Milk Biorad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pm40645185-229-59-65?v=Bio-Rad
Average 96 stars, based on 1 article reviews
04693116001 dctm protein assay kit ii biorad 5000112 blotting grade blocker non fat dry milk biorad - by Bioz Stars, 2026-08
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bio rad dc protein assay kit ii  (Bio-Rad)
93
Bio-Rad bio rad dc protein assay kit ii
Bio Rad Dc Protein Assay Kit Ii, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pmc03119499-113-6-6?v=Bio-Rad
Average 93 stars, based on 1 article reviews
bio rad dc protein assay kit ii - by Bioz Stars, 2026-08
93/100 stars
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ubiquitin antibody  (Proteintech)
96
Proteintech ubiquitin antibody
A) Day 1 EAK103 adults developed on L4440 (control) or cnnm-5 i at 25°C, zoomed in on the head region. Puncta indicate accumulation of mHTT tagged with YFP. Fluorescent images were taken using the GFP filter on the stereoscope at 63x. Scale bar indicates 500μm. B) MAH215 nematodes were imaged on Day 1 of adulthood after development at 25°C on L4440 (control) or cnnm-5 i. The green channel shows autophagosomes only (AP), and the red channel shows both autophagosomes and autolysosomes (AP + AL). Average autophagy levels in MAH215 L4440 or cnnm-5 i were quantified using ImageJ, p<0.05. Images in A & B are representative of three trials. C) Levels of ubiquitinated proteins in WT (lanes 1 & 2-biological replicates) and VC4126 (triple mutant strain that includes a nonsense mutation in cnnm-5 ) (lanes 3 & 3, technical replicates) on OP50 and EAK102 and EAK103 on control (L4440) ( EAK102 lanes 4-6, EAK103 lanes 10-12, biological triplicates) or cnnm-5 i ( EAK102 lanes 7-9, EAK103 lanes 13-15, biological triplicates). Beta-actin is shown as the loading control. The average <t>ubiquitin</t> signal was quantified and normalized to beta-actin. Two-tailed Student's t -test was performed to determine p value: N2 vs VC4126 p=0.65; EAK102 L4440 vs cnnm-5 i p=0.03 (*significant); EAK103 L4440 vs cnnm-5 i p=0.27. Fold change of normalized ubiquitin signal is based on comparison to the appropriate non- cnnm-5 -modified control (color matched). D) Survival curve of nematodes that survived each day of adulthood at 25°C. N2 and VC4126 were grown on OP50 , and EAK102 and EAK103 were developed and maintained on control (L4440) or cnnm-5 i. n=100 worms per condition. A chi-square test was performed to determine significance, *p<0.05. E) Percent of GMC101 nematodes that were paralyzed at adulthood Day 4 after 72 hours under heat stress (25°C) developed and grown on control (L4440) or cnnm-5 i. Each dot represents a separate trial (n>20 per trial). Bar within box represents the mean of all trials, and the whiskers represent standard error. One-tailed Student's t -test was performed to determine significance, *p=0.042.
Ubiquitin Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pmc12358017-79-22-24?v=Proteintech
Average 96 stars, based on 1 article reviews
ubiquitin antibody - by Bioz Stars, 2026-08
96/100 stars
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mini protean tgx gel bio rad  (Bio-Rad)
99
Bio-Rad mini protean tgx gel bio rad
A) Day 1 EAK103 adults developed on L4440 (control) or cnnm-5 i at 25°C, zoomed in on the head region. Puncta indicate accumulation of mHTT tagged with YFP. Fluorescent images were taken using the GFP filter on the stereoscope at 63x. Scale bar indicates 500μm. B) MAH215 nematodes were imaged on Day 1 of adulthood after development at 25°C on L4440 (control) or cnnm-5 i. The green channel shows autophagosomes only (AP), and the red channel shows both autophagosomes and autolysosomes (AP + AL). Average autophagy levels in MAH215 L4440 or cnnm-5 i were quantified using ImageJ, p<0.05. Images in A & B are representative of three trials. C) Levels of ubiquitinated proteins in WT (lanes 1 & 2-biological replicates) and VC4126 (triple mutant strain that includes a nonsense mutation in cnnm-5 ) (lanes 3 & 3, technical replicates) on OP50 and EAK102 and EAK103 on control (L4440) ( EAK102 lanes 4-6, EAK103 lanes 10-12, biological triplicates) or cnnm-5 i ( EAK102 lanes 7-9, EAK103 lanes 13-15, biological triplicates). Beta-actin is shown as the loading control. The average <t>ubiquitin</t> signal was quantified and normalized to beta-actin. Two-tailed Student's t -test was performed to determine p value: N2 vs VC4126 p=0.65; EAK102 L4440 vs cnnm-5 i p=0.03 (*significant); EAK103 L4440 vs cnnm-5 i p=0.27. Fold change of normalized ubiquitin signal is based on comparison to the appropriate non- cnnm-5 -modified control (color matched). D) Survival curve of nematodes that survived each day of adulthood at 25°C. N2 and VC4126 were grown on OP50 , and EAK102 and EAK103 were developed and maintained on control (L4440) or cnnm-5 i. n=100 worms per condition. A chi-square test was performed to determine significance, *p<0.05. E) Percent of GMC101 nematodes that were paralyzed at adulthood Day 4 after 72 hours under heat stress (25°C) developed and grown on control (L4440) or cnnm-5 i. Each dot represents a separate trial (n>20 per trial). Bar within box represents the mean of all trials, and the whiskers represent standard error. One-tailed Student's t -test was performed to determine significance, *p=0.042.
Mini Protean Tgx Gel Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pmc10542743__125_2023_5979_MOESM1_ESM-16-278-281?v=Bio-Rad
Average 99 stars, based on 1 article reviews
mini protean tgx gel bio rad - by Bioz Stars, 2026-08
99/100 stars
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lowrey method  (Bio-Rad)
97
Bio-Rad lowrey method
A) Day 1 EAK103 adults developed on L4440 (control) or cnnm-5 i at 25°C, zoomed in on the head region. Puncta indicate accumulation of mHTT tagged with YFP. Fluorescent images were taken using the GFP filter on the stereoscope at 63x. Scale bar indicates 500μm. B) MAH215 nematodes were imaged on Day 1 of adulthood after development at 25°C on L4440 (control) or cnnm-5 i. The green channel shows autophagosomes only (AP), and the red channel shows both autophagosomes and autolysosomes (AP + AL). Average autophagy levels in MAH215 L4440 or cnnm-5 i were quantified using ImageJ, p<0.05. Images in A & B are representative of three trials. C) Levels of ubiquitinated proteins in WT (lanes 1 & 2-biological replicates) and VC4126 (triple mutant strain that includes a nonsense mutation in cnnm-5 ) (lanes 3 & 3, technical replicates) on OP50 and EAK102 and EAK103 on control (L4440) ( EAK102 lanes 4-6, EAK103 lanes 10-12, biological triplicates) or cnnm-5 i ( EAK102 lanes 7-9, EAK103 lanes 13-15, biological triplicates). Beta-actin is shown as the loading control. The average <t>ubiquitin</t> signal was quantified and normalized to beta-actin. Two-tailed Student's t -test was performed to determine p value: N2 vs VC4126 p=0.65; EAK102 L4440 vs cnnm-5 i p=0.03 (*significant); EAK103 L4440 vs cnnm-5 i p=0.27. Fold change of normalized ubiquitin signal is based on comparison to the appropriate non- cnnm-5 -modified control (color matched). D) Survival curve of nematodes that survived each day of adulthood at 25°C. N2 and VC4126 were grown on OP50 , and EAK102 and EAK103 were developed and maintained on control (L4440) or cnnm-5 i. n=100 worms per condition. A chi-square test was performed to determine significance, *p<0.05. E) Percent of GMC101 nematodes that were paralyzed at adulthood Day 4 after 72 hours under heat stress (25°C) developed and grown on control (L4440) or cnnm-5 i. Each dot represents a separate trial (n>20 per trial). Bar within box represents the mean of all trials, and the whiskers represent standard error. One-tailed Student's t -test was performed to determine significance, *p=0.042.
Lowrey Method, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pmc07204889-113-9-11?v=Bio-Rad
Average 97 stars, based on 1 article reviews
lowrey method - by Bioz Stars, 2026-08
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frozen ez yeast transformation ii kit zymo research  (Zymo Research)
99
Zymo Research frozen ez yeast transformation ii kit zymo research
A) Day 1 EAK103 adults developed on L4440 (control) or cnnm-5 i at 25°C, zoomed in on the head region. Puncta indicate accumulation of mHTT tagged with YFP. Fluorescent images were taken using the GFP filter on the stereoscope at 63x. Scale bar indicates 500μm. B) MAH215 nematodes were imaged on Day 1 of adulthood after development at 25°C on L4440 (control) or cnnm-5 i. The green channel shows autophagosomes only (AP), and the red channel shows both autophagosomes and autolysosomes (AP + AL). Average autophagy levels in MAH215 L4440 or cnnm-5 i were quantified using ImageJ, p<0.05. Images in A & B are representative of three trials. C) Levels of ubiquitinated proteins in WT (lanes 1 & 2-biological replicates) and VC4126 (triple mutant strain that includes a nonsense mutation in cnnm-5 ) (lanes 3 & 3, technical replicates) on OP50 and EAK102 and EAK103 on control (L4440) ( EAK102 lanes 4-6, EAK103 lanes 10-12, biological triplicates) or cnnm-5 i ( EAK102 lanes 7-9, EAK103 lanes 13-15, biological triplicates). Beta-actin is shown as the loading control. The average <t>ubiquitin</t> signal was quantified and normalized to beta-actin. Two-tailed Student's t -test was performed to determine p value: N2 vs VC4126 p=0.65; EAK102 L4440 vs cnnm-5 i p=0.03 (*significant); EAK103 L4440 vs cnnm-5 i p=0.27. Fold change of normalized ubiquitin signal is based on comparison to the appropriate non- cnnm-5 -modified control (color matched). D) Survival curve of nematodes that survived each day of adulthood at 25°C. N2 and VC4126 were grown on OP50 , and EAK102 and EAK103 were developed and maintained on control (L4440) or cnnm-5 i. n=100 worms per condition. A chi-square test was performed to determine significance, *p<0.05. E) Percent of GMC101 nematodes that were paralyzed at adulthood Day 4 after 72 hours under heat stress (25°C) developed and grown on control (L4440) or cnnm-5 i. Each dot represents a separate trial (n>20 per trial). Bar within box represents the mean of all trials, and the whiskers represent standard error. One-tailed Student's t -test was performed to determine significance, *p=0.042.
Frozen Ez Yeast Transformation Ii Kit Zymo Research, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/10__7554_slash_elife__58313-399-303-309?v=Zymo+Research
Average 99 stars, based on 1 article reviews
frozen ez yeast transformation ii kit zymo research - by Bioz Stars, 2026-08
99/100 stars
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5000112 tumor dissociation kit miltenyi cat  (Miltenyi Biotec)
98
Miltenyi Biotec 5000112 tumor dissociation kit miltenyi cat
A) Day 1 EAK103 adults developed on L4440 (control) or cnnm-5 i at 25°C, zoomed in on the head region. Puncta indicate accumulation of mHTT tagged with YFP. Fluorescent images were taken using the GFP filter on the stereoscope at 63x. Scale bar indicates 500μm. B) MAH215 nematodes were imaged on Day 1 of adulthood after development at 25°C on L4440 (control) or cnnm-5 i. The green channel shows autophagosomes only (AP), and the red channel shows both autophagosomes and autolysosomes (AP + AL). Average autophagy levels in MAH215 L4440 or cnnm-5 i were quantified using ImageJ, p<0.05. Images in A & B are representative of three trials. C) Levels of ubiquitinated proteins in WT (lanes 1 & 2-biological replicates) and VC4126 (triple mutant strain that includes a nonsense mutation in cnnm-5 ) (lanes 3 & 3, technical replicates) on OP50 and EAK102 and EAK103 on control (L4440) ( EAK102 lanes 4-6, EAK103 lanes 10-12, biological triplicates) or cnnm-5 i ( EAK102 lanes 7-9, EAK103 lanes 13-15, biological triplicates). Beta-actin is shown as the loading control. The average <t>ubiquitin</t> signal was quantified and normalized to beta-actin. Two-tailed Student's t -test was performed to determine p value: N2 vs VC4126 p=0.65; EAK102 L4440 vs cnnm-5 i p=0.03 (*significant); EAK103 L4440 vs cnnm-5 i p=0.27. Fold change of normalized ubiquitin signal is based on comparison to the appropriate non- cnnm-5 -modified control (color matched). D) Survival curve of nematodes that survived each day of adulthood at 25°C. N2 and VC4126 were grown on OP50 , and EAK102 and EAK103 were developed and maintained on control (L4440) or cnnm-5 i. n=100 worms per condition. A chi-square test was performed to determine significance, *p<0.05. E) Percent of GMC101 nematodes that were paralyzed at adulthood Day 4 after 72 hours under heat stress (25°C) developed and grown on control (L4440) or cnnm-5 i. Each dot represents a separate trial (n>20 per trial). Bar within box represents the mean of all trials, and the whiskers represent standard error. One-tailed Student's t -test was performed to determine significance, *p=0.042.
5000112 Tumor Dissociation Kit Miltenyi Cat, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pm40885190-254-13-17?v=Miltenyi+Biotec
Average 98 stars, based on 1 article reviews
5000112 tumor dissociation kit miltenyi cat - by Bioz Stars, 2026-08
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protein concentration  (Bio-Rad)
99
Bio-Rad protein concentration
A) Day 1 EAK103 adults developed on L4440 (control) or cnnm-5 i at 25°C, zoomed in on the head region. Puncta indicate accumulation of mHTT tagged with YFP. Fluorescent images were taken using the GFP filter on the stereoscope at 63x. Scale bar indicates 500μm. B) MAH215 nematodes were imaged on Day 1 of adulthood after development at 25°C on L4440 (control) or cnnm-5 i. The green channel shows autophagosomes only (AP), and the red channel shows both autophagosomes and autolysosomes (AP + AL). Average autophagy levels in MAH215 L4440 or cnnm-5 i were quantified using ImageJ, p<0.05. Images in A & B are representative of three trials. C) Levels of ubiquitinated proteins in WT (lanes 1 & 2-biological replicates) and VC4126 (triple mutant strain that includes a nonsense mutation in cnnm-5 ) (lanes 3 & 3, technical replicates) on OP50 and EAK102 and EAK103 on control (L4440) ( EAK102 lanes 4-6, EAK103 lanes 10-12, biological triplicates) or cnnm-5 i ( EAK102 lanes 7-9, EAK103 lanes 13-15, biological triplicates). Beta-actin is shown as the loading control. The average <t>ubiquitin</t> signal was quantified and normalized to beta-actin. Two-tailed Student's t -test was performed to determine p value: N2 vs VC4126 p=0.65; EAK102 L4440 vs cnnm-5 i p=0.03 (*significant); EAK103 L4440 vs cnnm-5 i p=0.27. Fold change of normalized ubiquitin signal is based on comparison to the appropriate non- cnnm-5 -modified control (color matched). D) Survival curve of nematodes that survived each day of adulthood at 25°C. N2 and VC4126 were grown on OP50 , and EAK102 and EAK103 were developed and maintained on control (L4440) or cnnm-5 i. n=100 worms per condition. A chi-square test was performed to determine significance, *p<0.05. E) Percent of GMC101 nematodes that were paralyzed at adulthood Day 4 after 72 hours under heat stress (25°C) developed and grown on control (L4440) or cnnm-5 i. Each dot represents a separate trial (n>20 per trial). Bar within box represents the mean of all trials, and the whiskers represent standard error. One-tailed Student's t -test was performed to determine significance, *p=0.042.
Protein Concentration, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pm37543694-274-5-7?v=Bio-Rad
Average 99 stars, based on 1 article reviews
protein concentration - by Bioz Stars, 2026-08
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cell lines a431 cells atcc  (ATCC)
98
ATCC cell lines a431 cells atcc
A) Day 1 EAK103 adults developed on L4440 (control) or cnnm-5 i at 25°C, zoomed in on the head region. Puncta indicate accumulation of mHTT tagged with YFP. Fluorescent images were taken using the GFP filter on the stereoscope at 63x. Scale bar indicates 500μm. B) MAH215 nematodes were imaged on Day 1 of adulthood after development at 25°C on L4440 (control) or cnnm-5 i. The green channel shows autophagosomes only (AP), and the red channel shows both autophagosomes and autolysosomes (AP + AL). Average autophagy levels in MAH215 L4440 or cnnm-5 i were quantified using ImageJ, p<0.05. Images in A & B are representative of three trials. C) Levels of ubiquitinated proteins in WT (lanes 1 & 2-biological replicates) and VC4126 (triple mutant strain that includes a nonsense mutation in cnnm-5 ) (lanes 3 & 3, technical replicates) on OP50 and EAK102 and EAK103 on control (L4440) ( EAK102 lanes 4-6, EAK103 lanes 10-12, biological triplicates) or cnnm-5 i ( EAK102 lanes 7-9, EAK103 lanes 13-15, biological triplicates). Beta-actin is shown as the loading control. The average <t>ubiquitin</t> signal was quantified and normalized to beta-actin. Two-tailed Student's t -test was performed to determine p value: N2 vs VC4126 p=0.65; EAK102 L4440 vs cnnm-5 i p=0.03 (*significant); EAK103 L4440 vs cnnm-5 i p=0.27. Fold change of normalized ubiquitin signal is based on comparison to the appropriate non- cnnm-5 -modified control (color matched). D) Survival curve of nematodes that survived each day of adulthood at 25°C. N2 and VC4126 were grown on OP50 , and EAK102 and EAK103 were developed and maintained on control (L4440) or cnnm-5 i. n=100 worms per condition. A chi-square test was performed to determine significance, *p<0.05. E) Percent of GMC101 nematodes that were paralyzed at adulthood Day 4 after 72 hours under heat stress (25°C) developed and grown on control (L4440) or cnnm-5 i. Each dot represents a separate trial (n>20 per trial). Bar within box represents the mean of all trials, and the whiskers represent standard error. One-tailed Student's t -test was performed to determine significance, *p=0.042.
Cell Lines A431 Cells Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pm31178403-286-44-48?v=ATCC
Average 98 stars, based on 1 article reviews
cell lines a431 cells atcc - by Bioz Stars, 2026-08
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ripa lysis buffer thermo fisher scientific  (Thermo Fisher)
99
Thermo Fisher ripa lysis buffer thermo fisher scientific
A) Day 1 EAK103 adults developed on L4440 (control) or cnnm-5 i at 25°C, zoomed in on the head region. Puncta indicate accumulation of mHTT tagged with YFP. Fluorescent images were taken using the GFP filter on the stereoscope at 63x. Scale bar indicates 500μm. B) MAH215 nematodes were imaged on Day 1 of adulthood after development at 25°C on L4440 (control) or cnnm-5 i. The green channel shows autophagosomes only (AP), and the red channel shows both autophagosomes and autolysosomes (AP + AL). Average autophagy levels in MAH215 L4440 or cnnm-5 i were quantified using ImageJ, p<0.05. Images in A & B are representative of three trials. C) Levels of ubiquitinated proteins in WT (lanes 1 & 2-biological replicates) and VC4126 (triple mutant strain that includes a nonsense mutation in cnnm-5 ) (lanes 3 & 3, technical replicates) on OP50 and EAK102 and EAK103 on control (L4440) ( EAK102 lanes 4-6, EAK103 lanes 10-12, biological triplicates) or cnnm-5 i ( EAK102 lanes 7-9, EAK103 lanes 13-15, biological triplicates). Beta-actin is shown as the loading control. The average <t>ubiquitin</t> signal was quantified and normalized to beta-actin. Two-tailed Student's t -test was performed to determine p value: N2 vs VC4126 p=0.65; EAK102 L4440 vs cnnm-5 i p=0.03 (*significant); EAK103 L4440 vs cnnm-5 i p=0.27. Fold change of normalized ubiquitin signal is based on comparison to the appropriate non- cnnm-5 -modified control (color matched). D) Survival curve of nematodes that survived each day of adulthood at 25°C. N2 and VC4126 were grown on OP50 , and EAK102 and EAK103 were developed and maintained on control (L4440) or cnnm-5 i. n=100 worms per condition. A chi-square test was performed to determine significance, *p<0.05. E) Percent of GMC101 nematodes that were paralyzed at adulthood Day 4 after 72 hours under heat stress (25°C) developed and grown on control (L4440) or cnnm-5 i. Each dot represents a separate trial (n>20 per trial). Bar within box represents the mean of all trials, and the whiskers represent standard error. One-tailed Student's t -test was performed to determine significance, *p=0.042.
Ripa Lysis Buffer Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc+detergent+compatible+protein+assay+5000112+bio+rad/pm33894161-175-147-150?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
ripa lysis buffer thermo fisher scientific - by Bioz Stars, 2026-08
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Image Search Results


A) Day 1 EAK103 adults developed on L4440 (control) or cnnm-5 i at 25°C, zoomed in on the head region. Puncta indicate accumulation of mHTT tagged with YFP. Fluorescent images were taken using the GFP filter on the stereoscope at 63x. Scale bar indicates 500μm. B) MAH215 nematodes were imaged on Day 1 of adulthood after development at 25°C on L4440 (control) or cnnm-5 i. The green channel shows autophagosomes only (AP), and the red channel shows both autophagosomes and autolysosomes (AP + AL). Average autophagy levels in MAH215 L4440 or cnnm-5 i were quantified using ImageJ, p<0.05. Images in A & B are representative of three trials. C) Levels of ubiquitinated proteins in WT (lanes 1 & 2-biological replicates) and VC4126 (triple mutant strain that includes a nonsense mutation in cnnm-5 ) (lanes 3 & 3, technical replicates) on OP50 and EAK102 and EAK103 on control (L4440) ( EAK102 lanes 4-6, EAK103 lanes 10-12, biological triplicates) or cnnm-5 i ( EAK102 lanes 7-9, EAK103 lanes 13-15, biological triplicates). Beta-actin is shown as the loading control. The average ubiquitin signal was quantified and normalized to beta-actin. Two-tailed Student's t -test was performed to determine p value: N2 vs VC4126 p=0.65; EAK102 L4440 vs cnnm-5 i p=0.03 (*significant); EAK103 L4440 vs cnnm-5 i p=0.27. Fold change of normalized ubiquitin signal is based on comparison to the appropriate non- cnnm-5 -modified control (color matched). D) Survival curve of nematodes that survived each day of adulthood at 25°C. N2 and VC4126 were grown on OP50 , and EAK102 and EAK103 were developed and maintained on control (L4440) or cnnm-5 i. n=100 worms per condition. A chi-square test was performed to determine significance, *p<0.05. E) Percent of GMC101 nematodes that were paralyzed at adulthood Day 4 after 72 hours under heat stress (25°C) developed and grown on control (L4440) or cnnm-5 i. Each dot represents a separate trial (n>20 per trial). Bar within box represents the mean of all trials, and the whiskers represent standard error. One-tailed Student's t -test was performed to determine significance, *p=0.042.

Journal: microPublication Biology

Article Title: cnnm-5 knockdown improves proteostasis of mutant Huntingtin protein in C. elegans

doi: 10.17912/micropub.biology.001497

Figure Lengend Snippet: A) Day 1 EAK103 adults developed on L4440 (control) or cnnm-5 i at 25°C, zoomed in on the head region. Puncta indicate accumulation of mHTT tagged with YFP. Fluorescent images were taken using the GFP filter on the stereoscope at 63x. Scale bar indicates 500μm. B) MAH215 nematodes were imaged on Day 1 of adulthood after development at 25°C on L4440 (control) or cnnm-5 i. The green channel shows autophagosomes only (AP), and the red channel shows both autophagosomes and autolysosomes (AP + AL). Average autophagy levels in MAH215 L4440 or cnnm-5 i were quantified using ImageJ, p<0.05. Images in A & B are representative of three trials. C) Levels of ubiquitinated proteins in WT (lanes 1 & 2-biological replicates) and VC4126 (triple mutant strain that includes a nonsense mutation in cnnm-5 ) (lanes 3 & 3, technical replicates) on OP50 and EAK102 and EAK103 on control (L4440) ( EAK102 lanes 4-6, EAK103 lanes 10-12, biological triplicates) or cnnm-5 i ( EAK102 lanes 7-9, EAK103 lanes 13-15, biological triplicates). Beta-actin is shown as the loading control. The average ubiquitin signal was quantified and normalized to beta-actin. Two-tailed Student's t -test was performed to determine p value: N2 vs VC4126 p=0.65; EAK102 L4440 vs cnnm-5 i p=0.03 (*significant); EAK103 L4440 vs cnnm-5 i p=0.27. Fold change of normalized ubiquitin signal is based on comparison to the appropriate non- cnnm-5 -modified control (color matched). D) Survival curve of nematodes that survived each day of adulthood at 25°C. N2 and VC4126 were grown on OP50 , and EAK102 and EAK103 were developed and maintained on control (L4440) or cnnm-5 i. n=100 worms per condition. A chi-square test was performed to determine significance, *p<0.05. E) Percent of GMC101 nematodes that were paralyzed at adulthood Day 4 after 72 hours under heat stress (25°C) developed and grown on control (L4440) or cnnm-5 i. Each dot represents a separate trial (n>20 per trial). Bar within box represents the mean of all trials, and the whiskers represent standard error. One-tailed Student's t -test was performed to determine significance, *p=0.042.

Article Snippet: Worm bleach: 0.22M Sodium Hypochlorite and 0.786M Potassium Hydroxide in Millipore-filtered water Protein Quantification Assay Reagents (DC BCA): Bio Rad Catalog #5000112 Ubiquitin antibody: Proteintech Catalog #10201-2-AP Beta-actin antibody: Proteintech Catalog #81115-1-RR WesternSure Goat anti-rabbit HRP secondary: Licor Catalog #926-80011 SuperSignalTM West Femto Maximum Sensitivity Substrate: Fisher Catalog #34094 Sodium Azide : Fisher Catalog #S0489100G C. elegans strains: All strains were provided by the Caenorhabditis Genetics Center (CGC), which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440).

Techniques: Control, Mutagenesis, Ubiquitin Proteomics, Two Tailed Test, Comparison, Modification, One-tailed Test